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Nestin 抗体

NES 适用: 小鼠, 大鼠 WB, IF, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 4D4 (Rat-401) unconjugated
产品编号 ABIN112422
发货至: 中国
  • 抗原 See all Nestin (NES) 抗体
    Nestin (NES)
    适用
    • 129
    • 67
    • 46
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    小鼠, 大鼠
    宿主
    • 103
    • 33
    • 4
    • 2
    • 1
    小鼠
    克隆类型
    • 108
    • 35
    单克隆
    标记
    • 61
    • 10
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    • 4
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    • 4
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    • 4
    • 4
    • 3
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    This Nestin antibody is un-conjugated
    应用范围
    • 78
    • 50
    • 48
    • 47
    • 40
    • 39
    • 38
    • 29
    • 24
    • 14
    • 7
    • 6
    • 2
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    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    This antibody recognizes Mouse and Rat Nestin and does not recognizes Human Nestin.
    纯化方法
    Ig Fraction
    免疫原
    Nestin purified from embryonic rat spinal cord. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
    克隆位点
    4D4 (Rat-401)
    亚型
    IgG
    Top Product
    Discover our top product NES Primary Antibody
  • 应用备注
    Western Blot: reacts with a band at 200-220 kDa in reducing gels of newborn rat or mousecell extracts, For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol and4.6 % SDS, Membranes should be blocked with milk or BSA, 5 % PAGE gels are suggested. Immunohistochemistry on frozen and paraffin sections: 1/20 - 1/200, We recommendperfusing tissues with 4 % paraformaldehyde at pH 7.4 for light microscopy or with either4 % paraformaldehyde at pH 10.0 or 4 % paraformaldehyde with 0.1 % glutaraldehyde at pH 7.4 for EM, For Immunocytochemistry we recommend using cells fixed in 4 %paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    TRIS buffered saline, 0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    Nestin (NES)
    别名
    Nestin (NES 产品)
    别名
    paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody
    背景
    Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.Synonyms: NES, Nbla00170
    基因ID
    18008
    NCBI登录号
    NP_057910
    UniProt
    Q6P5H2
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