TNFRSF1B 抗体 (Biotin)
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- 抗原 See all TNFRSF1B 抗体
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TNFRSF1B antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography
- 免疫原
- Partially purified preparations of TNF binding proteins
- 克隆位点
- Utr1
- 亚型
- IgG1
- Top Product
- Discover our top product TNFRSF1B Primary Antibody
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anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) antibody
TNFRSF1B 适用: 人, 小鼠 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 2H11C2 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) antibodyTNFRSF1B 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 7G8B6 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (C-Term) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (N-Term) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 30-250) antibodyTNFRSF1B 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (C-Term) antibodyTNFRSF1B 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 1-461) antibodyTNFRSF1B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibodyTNFRSF1B 适用: 人 WB, ELISA, IHC, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibody (Biotin)TNFRSF1B 适用: 小鼠 WB, FACS, IP, Func, IA 宿主: 大鼠 Monoclonal HM102 Biotin
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- 应用备注
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Immunohistochemistry on Frozen Sections: 1 μg/mL (1/400) andImmunohistochemistry on Paraffin Sections: 10 μg/mL (1/40). Pretreatment not necessary. Recommended Positive Control: Human tonsil. Has also been described to work in FACS, Western Blots and Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer’s hemalum.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 0.5 mL distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- PBS, pH 7.2, containing 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!.
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- 抗原
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
- 别名
- CD120b / TNFR2 (TNFRSF1B 产品)
- 背景
- Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor
- 基因ID
- 9606
- UniProt
- P20333
- 途径
- NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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