S100A8 抗体 (C-Term)
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- 抗原 See all S100A8 抗体
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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抗原表位
- C-Term
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适用
- 人, 大鼠, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This S100A8 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB)
- 特异性
- Clone S13.67 identifies MRP8: The antibody is useful in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. This clone also stains cells in rat spleen, indiating significant cross reactivity with the corresponding Rat MRP8. Antigen Distribution on Isolated Cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Antigen Distribution on Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages increasingly synthesise MRP-8 during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/MRP14.
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Cultured Human monocytes. Remarks: Antigen: MRP8. Epitope: Suspected in the N- or C-terminal Domain.
- 克隆位点
- S13-67
- 亚型
- IgG1
- Top Product
- Discover our top product S100A8 Primary Antibody
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anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-89) antibody
S100A8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-89) antibodyS100A8 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 犬, Cat, 猴, 马, 豚鼠, Baboon, 山羊 IHC, StM 宿主: 兔 Monoclonal MAC3157R unconjugated Recombinant Antibody
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 犬, Cat, 猴, 马, 豚鼠, Baboon, 山羊 IHC, StM 宿主: 小鼠 Monoclonal rMAC3781 unconjugated Recombinant Antibody
anti-S100 Calcium Binding Protein A8 (S100A8) (C-Term) antibodyS100A8 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA. Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/100-1/200). Immunohistochemistry on Paraffin Sections: 4 μg/mL (1/50). Pre-treatment for antigenretrieval not required. Has been described to work in FACS and Dot blots. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 0.5 mL distilled water.
- 浓度
- 0.2 mg/mL
- 缓冲液
- Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.05 % (v/v) Kathon CG as preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- Do Not Use Sodium Azide as preservative.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
- 别名
- S100A8 / Calgranulin-A / MRP8 (S100A8 产品)
- 别名
- 60B8AG antibody, CAGA antibody, CFAG antibody, CGLA antibody, CP-10 antibody, L1Ag antibody, MA387 antibody, MIF antibody, MRP8 antibody, NIF antibody, P8 antibody, Mrp8 antibody, S100A8 antibody, 60B8Ag antibody, AI323541 antibody, B8Ag antibody, CFAg antibody, Caga antibody, p8 antibody, MRP-8 antibody, S100 calcium binding protein A8 antibody, S100 calcium binding protein A8 (calgranulin A) antibody, S100A8 antibody, S100a8 antibody
- 背景
- MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A
- 基因ID
- 9606
- UniProt
- P05109
- 途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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