S100A8 抗体 (S100 Calcium Binding Protein A8) (Biotin)

Details for Product anti-S100A8 Antibody No. ABIN111891, 供应商: Log in to see
抗原
  • EWWD
  • MRP8
  • WW
  • 60B8AG
  • CAGA
  • CFAG
  • CGLA
  • CP-10
  • L1Ag
  • MA387
  • MIF
  • NIF
  • P8
  • Mrp8
  • S100A8
  • 60B8Ag
  • AI323541
  • B8Ag
  • CFAg
  • Caga
  • p8
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 11
  • S100 calcium binding protein A8
  • S100 calcium binding protein A8 (calgranulin A)
  • ABCC11
  • S100A8
  • S100a8
适用

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小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
This S100A8 antibody is conjugated to Biotin
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应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 Cultured Human monocytes. Remarks: The antigen is MRP8, the epitope is suspected in the central portion of the peptide.
克隆位点 8-5C2
亚型 IgG1
特异性 This Monoclonal antibody 8-5C2 detects a very distinct epitope in the central part of the molecule, in between the two Ca2+-binding domains (different from the S 13.67 epitope). This antibody identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. It is useful for the identification of the 8 kDa subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. Antigen Distribution Isolated Cells: The antigen is found in granulocytes and monocytes but not in other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukemia stain positive. Tissue Sections: In tissues MRP8 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP8 increasingly during the late stages of inflammation. A low MRP8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis or chronic rejection after allograft transplantation, MRP8 and MRP14 positive cells consist of different subpopulations. The antibody reacts with Bovine spleen but does not react with Rat tissues. It shows a diffuse reaction with Swine spleen.
交叉反应 (详细) Species reactivity (tested):Human: MRP8 in stimulated monocytes and macrophages in late phase or chronic inflammation.
纯化方法 Protein A Chromatography
别名 S100A8 / Calgranulin-A / MRP8 (S100A8 Antibody 摘要)
背景 Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A
基因ID 9606
UniProt P05109
研究领域 Cell Cycle
途径 Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity
应用备注 ELISA. Flow Cytometry. Immunohistochemistry (IHC) on: Frozen Sections: 2 μg/mL (1/100). Paraffin Sections: 5-10 μg/mL (1/20-1/50). No pretreatment for antigen retrieval necessary,microwave treatment in 0.01M citrate pH 6.0 may enhance the reactivity. Suggested positive control: Human tonsil. Has been described to work Dot blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 0.5 mL distilled water (This stock solution contains 0.2 mg/mL IgG).
浓度 0.2 mg/mL
缓冲液 PBS, pH 7.2, 0.05 % (v/v) Kathon, 5 mg/mL BSA
储存液 Thimerosal (Merthiolate)
注意事项 This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Dilute only prior to immediate use
储存条件 4 °C
储存方法 Store the antibody at 2-8 °C before opening. DO NOT FREEZE! This product is stable at 2-8 °C as an undiluted liquid. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
有引用在: Wong, Zhou, Li, Tong, Zhao, Li, Yu, Koh, Beuerman: "Proteomic profiling of inflammatory signaling molecules in the tears of patients on chronic glaucoma medication." in: Investigative ophthalmology & visual science, Vol. 52, Issue 10, pp. 7385-91, 2011 (PubMed).

Mortezai, Harder, Schnabel, Moors, Gauly, Schlüter, Wagener, Buck: "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids." in: Journal of proteome research, Vol. 9, Issue 12, pp. 6126-34, 2010 (PubMed).

Rosenberger, Thorey, Werner, Boukamp: "A novel regulator of telomerase. S100A8 mediates differentiation-dependent and calcium-induced inhibition of telomerase activity in the human epidermal keratinocyte line HaCaT." in: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6126-35, 2007 (PubMed).

背景 Wen, Xu, Mais, Goldman, McDonnell: "The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8356-64, 1994 (PubMed).

Teigelkamp, Bhardwaj, Roth, Meinardus-Hager, Karas, Sorg: "Calcium-dependent complex assembly of the myeloic differentiation proteins MRP-8 and MRP-14." in: The Journal of biological chemistry, Vol. 266, Issue 20, pp. 13462-7, 1991 (PubMed).

Mues, Brisse, Zwadlo, Themann, Bender, Sorg: "Phenotyping of macrophages with monoclonal antibodies in endomyocardial biopsies as a new approach to diagnosis of myocarditis." in: European heart journal, Vol. 11, Issue 7, pp. 619-27, 1990 (PubMed).

Delabie, de Wolf-Peeters, van den Oord, Desmet: "Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study." in: Clinical and experimental immunology, Vol. 81, Issue 1, pp. 123-6, 1990 (PubMed).

Murao, Collart, Huberman: "A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases." in: The Journal of biological chemistry, Vol. 264, Issue 14, pp. 8356-60, 1989 (PubMed).

Zwadlo, Brüggen, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues." in: Clinical and experimental immunology, Vol. 72, Issue 3, pp. 510-5, 1988 (PubMed).

Odink, Cerletti, Brüggen, Clerc, Tarcsay, Zwadlo, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis." in: Nature, Vol. 330, Issue 6143, pp. 80-2, 1987 (PubMed).

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