山羊 anti-小鼠 IgG3 (Heavy Chain) Antibody (Atto 550) - Preadsorbed
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- 抗原 See all IgG3 products
- IgG3
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Atto 550
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - 交叉反应
- 小鼠
- 产品特性
- ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Mouse IgG3 heavy chain
Immunogen Type: Native Protein
- 亚型
- IgG3
- Labeling Ratio
- 2.5
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Goat anti-Mouse IgG3 antibody (PE-Cy7) - Preadsorbed
ELISA, FACS, FLISA Polyclonal IgG PE-Cy7
Goat anti-Mouse IgG3 antibody (SPRD) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG SPRD
Rat anti-Mouse IgG3 antibody (PE)ELISA, FACS, FLISA Monoclonal SB76b IgG2a PE
Goat anti-Mouse IgG3 antibody (HRP)ELISA, FLISA Polyclonal IgG HRP
Goat anti-Mouse IgG3 (Heavy Chain) antibody (HRP)ELISA, WB, IHC Polyclonal IgG HRP
Goat anti-Mouse IgG3 antibodyELISA, FLISA Polyclonal IgG unconjugated
Goat anti-Mouse IgG3 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Rat anti-Mouse IgG3 antibodyELISA, FACS, FLISA Monoclonal SB76b IgG2a unconjugated
Goat anti-Mouse IgG3 antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
Goat anti-Mouse IgG3 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
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- 应用备注
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Immunohistochemistry Dilution: >1:5,000
Application Note: ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Anti-Mouse IgG3 secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Flow Cytometry Dilution: 1:500 - 1:2,500
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 说明
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The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG3
- Abstract
- IgG3 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-MOUSE IgG3 Antibody ATTO550 Conjugation, goat anti-MOUSE IgG3 ATTO 550 Conjugated Antibody
Background: Anti-Mouse IgG3 ATTO 550 Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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