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山羊 anti-小鼠 IgG1 (Heavy Chain) Antibody (Atto 532) - Preadsorbed

WB, FLISA, FM Polyclonal IgG1 Atto 532
产品编号 ABIN1118511
发货至: 中国
  • 抗原 See all IgG1 products
    IgG1
    抗原表位
    • 41
    • 26
    • 17
    • 6
    • 5
    • 3
    • 1
    • 1
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    Heavy Chain
    适用
    • 84
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    小鼠
    宿主
    • 63
    • 39
    • 35
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    山羊
    克隆类型
    • 96
    • 54
    多克隆
    标记
    • 44
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    Atto 532
    应用范围
    • 76
    • 56
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    • 42
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    • 18
    • 12
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    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG.
    Specificity was confirmed by ELISA at less than 0.5 % of target signal.
    交叉反应
    小鼠
    产品特性
    ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG1 heavy chain

    Immunogen Type: Native Protein

    亚型
    IgG1
    Labeling Ratio
    2.5
  • 应用备注

    Application Note: ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    说明

    The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid cycles of freezing and thawing.
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution.
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG1
    Abstract
    IgG1 产品
    物质类
    Antibody
    背景

    Synonyms: Goat anti-mouse IgG1 antibody ATTO488 conjugation, goat anti-mouse IgG1 (gamma 1) ATTO 488 conjugated antibody

    Background: Anti-Mouse IgG1 ATTO 532 Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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