山羊 anti-小鼠 IgG2b (Heavy Chain) Antibody (Atto 532) - Preadsorbed
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- 抗原 See all IgG2b products
- IgG2b
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Atto 532
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 特异性
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - 交叉反应
- 小鼠
- 产品特性
- ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Mouse IgG2b heavy chain
Immunogen Type: Native Protein
- 亚型
- IgG2b
- Labeling Ratio
- 3.0
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Goat anti-Mouse IgG2b (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG2b (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG2b (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG2b antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Rat anti-Mouse IgG2b antibody (PE)ELISA, FACS, FLISA Monoclonal SB74g IgG2b PE
Goat anti-Mouse IgG2b antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2b antibodyELISA, FLISA Polyclonal IgG unconjugated
Goat anti-Mouse IgG2b (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Mouse IgG2b (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rat anti-Mouse IgG2b antibodyELISA, FACS, FLISA Monoclonal SB74g IgG2b unconjugated
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- 应用备注
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Application Note: Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: >1:20,000
Flow Cytometry Dilution: 1:500 - 1:2,500
Western Blot Dilution: 1:4,000 - 1:20,000
IF Microscopy Dilution: >1:5,000
- 说明
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The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG2b
- Abstract
- IgG2b 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-MOUSE IgG2b Antibody ATTO532 Conjugation, goat anti-Mouse IgG2b (gamma 2b) ATTO 532 Conjugated Antibody
Background: Anti-Mouse IgG2b ATTO 532 Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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