MAP2 抗体 (MAP-2) (Biotin)

Details for Product anti-MAP-2 Antibody No. ABIN111292, 供应商: Log in to see
抗原
  • p67
  • p67eIF2
  • MAP2A
  • MAP2B
  • MAP2C
  • MAP-2
  • Mtap-2
  • Mtap2
  • MAP2R
  • xmap2
  • MGC53131
  • a730034c02
  • microtubule-associated protein 2
  • methionyl aminopeptidase 2
  • microtubule-associated protein 2-like
  • MAP2
  • METAP2
  • Map2
  • map2
  • LOC100339229
适用
小鸡, 牛, 人, 小鼠, Quail, 大鼠, 非洲爪蟾
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12
9
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1
宿主
小鼠
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4
克隆类型 (克隆位点)
单克隆 ()
标记
This MAP2 antibody is conjugated to Biotin
18
15
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1
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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100
68
46
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26
26
13
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6
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2
1
选项
Supplier
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Supplier Product No.
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免疫原 Bovine brain microtubule associated protein 2.
克隆位点 AP20
亚型 IgG1
特异性 Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendrites and cell bodies of neurons.
纯化方法 Protein G chromatography
背景 Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker
基因ID 4133
NCBI登录号 NP_002365
UniProt P11137
研究领域 Cytoskeleton
应用备注 Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. WesternBlotting: 1-2 μg/mL for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mMcitrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. ]Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant braintissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 0.1 mg/mL
缓冲液 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0 % BSA and 0.09 % sodium azide as a preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
背景 Kalcheva, Albala, Binder, Shafit-Zagardo: "Localization of specific epitopes on human microtubule-associated protein 2." in: Journal of neurochemistry, Vol. 63, Issue 6, pp. 2336-41, 1994 (PubMed).

Lewis, Ivanov, Lee, Cowan: "Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau." in: Nature, Vol. 342, Issue 6249, pp. 498-505, 1990 (PubMed).

Peng, Binder, Black: "Biochemical and immunological analyses of cytoskeletal domains of neurons." in: The Journal of cell biology, Vol. 102, Issue 1, pp. 252-62, 1986 (PubMed).

Binder, Frankfurter, Rebhun: "Differential localization of MAP-2 and tau in mammalian neurons in situ." in: Annals of the New York Academy of Sciences, Vol. 466, pp. 145-66, 1986 (PubMed).

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