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KRT20 抗体 (FITC)

KRT20 适用: 人, 大鼠, Pig IHC (p), IF, IHC (fro), FACS 宿主: 小鼠 Monoclonal KS20-10 FITC
产品编号 ABIN111170
发货至: 中国
  • 抗原 See all KRT20 抗体
    KRT20 (Keratin 20 (KRT20))
    适用
    • 91
    • 29
    • 27
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    人, 大鼠, Pig
    宿主
    • 62
    • 30
    • 3
    • 1
    • 1
    小鼠
    克隆类型
    • 52
    • 45
    单克隆
    标记
    • 63
    • 12
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This KRT20 antibody is conjugated to FITC
    应用范围
    • 68
    • 46
    • 25
    • 15
    • 14
    • 12
    • 11
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
    特异性
    This Monoclonal antibody represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide Recognized: Protein IT (keratin K20, Mr 46 000, also designated Cytokeratin 20). Tested Reactivities on Cultured Cell Lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
    无交叉反应
    小鼠
    交叉反应 (详细)
    Species reactivity (tested):Human, Porcine (Pig) and Rat.
    纯化方法
    Affinity Chromatography
    免疫原
    Electrophoretically purified Keratin K 20 from Human intestinal mucosa.
    克隆位点
    KS20-10
    亚型
    IgG1
    Top Product
    Discover our top product KRT20 Primary Antibody
  • 应用备注
    FACS. Cytological Material. Immunohistochemistry on Frozen Sections. . Immunohistochemistry on Paraffin sections (Only after microwave treatment). Working Dilution: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8 °C.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Mușină, Zlei, Mentel, Scripcariu, Ștefan, Aniţei, Filip, Radu, Gavrilescu, Panuţa, Buna-Arvinte, Cordun, Predescu, Scripcariu, Huţanu: "Evaluation of circulating tumor cells in colorectal cancer using flow cytometry." in: The Journal of international medical research, Vol. 49, Issue 9, pp. 300060520980215, (2021) (PubMed).

  • 抗原
    KRT20 (Keratin 20 (KRT20))
    别名
    Cytokeratin 20 (KRT20 产品)
    别名
    KRT20 antibody, CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, Krt21 antibody, 9030623C06Rik antibody, keratin 20 antibody, KRT20 antibody, Krt20 antibody
    背景
    Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
    基因ID
    54474
    NCBI登录号
    NP_061883
    UniProt
    P35900
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