KRT20 抗体
(2 references)-
- 抗原 See all KRT20 抗体
- KRT20 (Keratin 20 (KRT20))
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适用
- 人, 大鼠, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This KRT20 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- IT-Ks 20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reactivities on cultured cell lines (tested so far): HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
- 纯化方法
- Protein A affinity chromatography
- 免疫原
- Electrophoretically purified Keratin K20 from human intestinal mucosa
- 克隆位点
- KS20-10
- 亚型
- IgG1
- Top Product
- Discover our top product KRT20 Primary Antibody
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anti-Keratin 20 (KRT20) antibody
KRT20 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 1-424) antibodyKRT20 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2G3-1C8 unconjugated
anti-Keratin 20 (KRT20) (AA 245-424) antibodyKRT20 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal KRT20-1992 unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal KRT20-1991 unconjugated
anti-Keratin 20 (KRT20) (AA 1-266) antibodyKRT20 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4A2 unconjugated
anti-Keratin 20 (KRT20) antibodyKRT20 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal KRT20-3129R unconjugated Recombinant Antibody
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal KRT20-3145 unconjugated
anti-Keratin 20 (KRT20) (AA 1-150) antibodyKRT20 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2C7B9 unconjugated
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- 应用备注
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Immunohistochemistry on frozen tissue. Immunohistochemistry on paraffin embedded sections (only after after microwavetreatment). Dilute 1/10 with PBS for immunohistochemistry. Incubation time: 1h at RT, orover night at 4C. Western blotting. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL distilled water.
- 缓冲液
- Final solution contains 0.09 % NaN3, 0.5 % BSA in PBS buffer, pH 7.4.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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: "The detection of EpCAM(+) and EpCAM(-) circulating tumor cells." in: Scientific reports, Vol. 5, pp. 12270, (2015) (PubMed).
: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).
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: "The detection of EpCAM(+) and EpCAM(-) circulating tumor cells." in: Scientific reports, Vol. 5, pp. 12270, (2015) (PubMed).
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- 抗原
- KRT20 (Keratin 20 (KRT20))
- 别名
- Cytokeratin 20 (KRT20 产品)
- 别名
- KRT20 antibody, CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, Krt21 antibody, 9030623C06Rik antibody, keratin 20 antibody, KRT20 antibody, Krt20 antibody
- 背景
- Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
- 基因ID
- 54474
- NCBI登录号
- NP_061883
- UniProt
- P35900
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