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TUBB3 抗体 (N-Term)

Name: TUBB3 抗体 (N-Term)
SKU: ABIN110982
Availability: OutOfStock

TUBB3 适用: Vicia faba WB, FACS, IHC (p), IF, IHC (fro), EIA 宿主: 小鼠 Monoclonal TU-20 unconjugated

(3 references)

产品编号 ABIN110982

发货至: 中国

Datasheet as PDF

抗原 See all TUBB3 抗体

TUBB3 (Tubulin, beta 3 (TUBB3))
抗原表位
21

16

15

12

7

6

5

3

2

2

2

2

1

1

1

1

1

1

1

1

1
N-Term
适用
126

70

63

21

7

6

5

4

4

3

3

2

2

2

2

2

1

1

1

1
Vicia faba
宿主
80

49

4

1

1
小鼠
Compatible Secondaries
克隆类型
71

64
单克隆
标记
80

14

7

5

3

3

3

3

3

3

3

2

1

1

1

1

1

1
This TUBB3 antibody is un-conjugated
应用范围
109

41

40

36

29

26

25

15

15

14

12

6

5

4

3
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

特异性

This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of Human class III β-tubulin specific for neurones. The antibody is a highly specific marker for neuronal tissue. TU-20 is very useful for the detection of microtubule structures on fixed cells. This Monoclonal antibody TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).

纯化方法

Ammonium Sulphate and Caprylic Acid Precipitation

免疫原

Peptide (C) 441-448 coupled to Maleimide-activated Keyhole Limpet Hemocyanin via Cysteine added to the N-terminus of the neuron-specific peptide.

克隆位点

TU-20

亚型

IgG1

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anti-Tubulin, beta 3 (TUBB3) (N-Term) antibody
TUBB3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal TU-20 unconjugated

anti-Tubulin, beta 3 (TUBB3) (N-Term) antibody (FITC)
TUBB3 适用: 人, 小鼠, 大鼠, 犬, Pig FACS, ICC 宿主: 小鼠 Monoclonal TU-20 FITC

anti-Tubulin, beta 3 (TUBB3) (AA 443-450) antibody
TUBB3 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 8E3A7 unconjugated

anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibody
TUBB3 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21098 unconjugated

anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibody
TUBB3 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 2-00E-09 unconjugated

anti-Tubulin, beta 3 (TUBB3) (AA 401-450) antibody
TUBB3 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 小鼠 Monoclonal unconjugated

应用备注

Flow cytometry. ELISA. Western Blotting: 1-2 μg/mL, 90 min. Positive Control: Porcine brain lysate. Negative Control: HPB-ALL peripheral blood leukemia cell line. Sample Preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 10 μg/mLStaining technique: Standard ABC technique (DAB+). Pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT or hightemperature citrate buffer antigen retrieval. Positive tissue: Neuronal tissue. Immunocytochemistry: FITC conjugate: 3 μg/mL. Positive Material: Neuro2a mouse neuroblastoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, pH ~7.4, 15 mM Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).
抗原

TUBB3 (Tubulin, beta 3 (TUBB3))

别名

TUBB3 / TUBB4 (TUBB3 产品)

背景

The beta-III Tubulin isotype is present dominantly in cells of neuronal origin and it is one of the earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The neuron-associated class III beta-tubulin isotype is most abundant in cells of neuronal origin but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.Synonyms: Tubulin beta-3 chain, Tubulin beta-4, Tubulin beta-III

基因ID

10381

NCBI登录号

NP_001184110

UniProt

Q13509

途径

Microtubule Dynamics, M Phase