HA-标记 抗体

ABIN1107475 产品详细信息, 供应商: Log in to see
抗原
适用
标记
447
32
18
8
2
1
1
1
宿主
小鼠
213
180
40
34
20
3
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
45
31
24
10
10
9
9
9
8
8
7
7
6
6
6
6
6
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
392
201
122
118
85
81
76
40
15
13
11
7
7
5
4
4
4
4
3
3
2
2
1
1
1
1
1
选项
Supplier
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Supplier Product No.
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免疫原 A synthetic peptide (YPYDVPDYA) from Influenza Hemagglutinin epitope coupled to KLH.
序列 YPYDVPDYA
克隆位点 HA-C5
亚型 IgG3
产品特性 Synonyms: Hemagglutinin Tag, HA-Tag, HA Tag
纯化方法 Affinity Chromatography
别名 HA Epitope Tag (YPYDVPDYA)
物质类 Tag
背景 Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
应用备注 Western blotting (1/1,000). Immunoprecipitation. Immunostaining (1/200). Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4, 0.05 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN1107475) anti-HA-Tag antibody
Immunofluorescence (IF) image for anti-HA-Tag antibody (ABIN1107475) anti-HA-Tag antibody (Image 2)
背景 Hu, Luo, Ji, Chulu, Chang, Shieh, Wang, Liu: "Dual expression of the HA protein of H5N2 avian influenza virus in a baculovirus system." in: Journal of virological methods, Vol. 135, Issue 1, pp. 43-8, 2006 (PubMed).

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