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Myc 标记 抗体 (Agarose Beads)

ABIN1105586 产品详细信息, 供应商: Log in to see
抗原
适用
标记
212
36
14
10
6
5
3
1
1
宿主
小鼠
160
60
17
16
3
2
1
克隆类型 (克隆位点)
单克隆 ()
标记
Agarose Beads
27
23
14
8
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunoprecipitation (IP)
216
116
77
53
48
34
22
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
Supplier
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免疫原 GST-6myc-Tag fusion protein
序列 EQKLISEEDL
克隆位点 PL14
亚型 IgG1
特异性 This antibody recognizes Myc-Tag peptide sequence (EQKLISEEDL).
纯化方法 Protein A agarose
别名 C-Myc Epitope Tag
物质类 Tag
背景 Epitope tagging is a widely accepted technique that fuses an epitope-containing peptide to a target protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VAV-G- Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
基因ID 4609
NCBI登录号 NP_002458
UniProt P01106
研究领域 Transcription Factors, Cancer, Cell Cycle
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 200 μg of anti-Myc Tag monoclonal antibody covalently coupled to 200 μL of agarose gel and provided as a 50 % gel slurry suspended in PBS containing preservative (0.09 % sodium azide) for a total volume of 400 μL
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store at 2 - 8 °C.
厂商提供的图像
Western Blotting (WB) image for anti-Myc 标记 抗体 (Agarose Beads) (ABIN1105586) anti-Myc Tag antibody (Agarose Beads)
背景 Hirota, Daitoku, Matsuzaki, Araya, Yamagata, Asada, Sugaya, Fukamizu: "Hepatocyte nuclear factor-4 is a novel downstream target of insulin via FKHR as a signal-regulated transcriptional inhibitor." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 13056-60, 2003 (PubMed).

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002 (PubMed).

Bae, Ahn, Jeong, Bae, Lee, Bae, Park, Kim, Kim: "Jab1 interacts directly with HIF-1alpha and regulates its stability." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 9-12, 2002 (PubMed).

Tojo, Matsuzaki, Minami, Honda, Yasuda, Chiba, Saya, Fujii-Kuriyama, Nakao: "The aryl hydrocarbon receptor nuclear transporter is modulated by the SUMO-1 conjugation system." in: The Journal of biological chemistry, Vol. 277, Issue 48, pp. 46576-85, 2002 (PubMed).

"In Celebration of 100 Years of Publication." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 1, 2001 (PubMed).

Mukai, Hachiya, Shoji-Hoshino, Kimura, Nadano, Suvanto, Hanaoka, Li, Irie, Greene, Sato: "NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR." in: The Journal of biological chemistry, Vol. 275, Issue 23, pp. 17566-70, 2000 (PubMed).