Myc 标记 抗体

ABIN1105584 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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选项
Supplier
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免疫原 GST-6myc-Tag fusion protein
序列 EQKLISEEDL
克隆位点 PL14
亚型 IgG1
特异性 This antibody can be used for epitope-tagging using the amino acid sequence EQKLISEEDL.
纯化方法 Protein-A Sepharose
别名 C-Myc Epitope Tag
物质类 Tag
背景 Epitope tagging is a widely accepted technique that fuses an epitope peptide to a protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follows, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which correspond to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
基因ID 4609
NCBI登录号 NP_002458
UniProt P01106
研究领域 Transcription Factors, Cancer, Cell Cycle
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
缓冲液 PBS containing 50 % glycerol, without preservatives
储存液 Without preservative
注意事项 Avoid repeated freezing and thawing.
储存条件 -20 °C
储存方法 Store (in aliquots) at -20 °C.
背景 Heald, McLoughlin, McKeon: "Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase." in: Cell, Vol. 74, Issue 3, pp. 463-74, 1993 (PubMed).

Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

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