His 标记 抗体

ABIN1105145 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主
小鼠
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1
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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应用范围
Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 Synthetic peptide 6 His
序列 HHHHHH
克隆位点 6C4
亚型 IgG1
纯化方法 Protein A agarose
别名 6xHistidine Epitope Tag
物质类 Tag
背景 A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The His-Tag fusion protein expression system is commonly used because the 6 x His-Tag on the recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
缓冲液 PBS containing 50 % glycerol, pH 7.2, without preservatives
储存液 Without preservative
注意事项 Avoid repeated freezing and thawing.
储存条件 -20 °C
储存方法 Store (in aliquots) at -20 °C.
厂商提供的图像
Western Blotting (WB) image for anti-His Tag antibody (ABIN1105145) anti-His Tag antibody
背景 Porath: "Immobilized metal ion affinity chromatography." in: Protein expression and purification, Vol. 3, Issue 4, pp. 263-81, 1992 (PubMed).

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