His 标记 抗体

ABIN1105141 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
24
22
14
3
2
宿主
小鼠
434
184
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
59
45
36
15
14
12
11
10
10
10
10
9
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4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
554
386
166
106
99
77
69
26
13
11
9
7
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6
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1
1
1
1
1
1
1
1
1
1
选项
Supplier
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Supplier Product No.
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免疫原 Synthetic peptide containing the His epitope 6x His (H-H-H-H-H-H).
序列 HHHHHH
克隆位点 HIS-H8
亚型 IgG2b
纯化方法 Affinity Chromatography.
别名 6xHistidine Epitope Tag
物质类 Tag
背景 Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 -20 °C
储存方法 Store the antibody (in aliquots) at -20 °C.
厂商提供的图像
Western Blotting (WB) image for anti-His Tag antibody (ABIN1105141) anti-His Tag antibody
Immunofluorescence (IF) image for anti-His Tag antibody (ABIN1105141) anti-His Tag antibody (Image 2)
背景 Xu, Liu, He: "Expression and purification of soluble human programmed death-1 in Escherichia coli." in: Cellular & molecular immunology, Vol. 3, Issue 2, pp. 139-43, 2006 (PubMed).

Terpe: "Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems." in: Applied microbiology and biotechnology, Vol. 60, Issue 5, pp. 523-33, 2003 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).

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