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RFP 抗体 (Red Fluorescent Protein) (Biotin)

Details for Product anti-RFP Antibody No. ABIN1103973, 供应商: Log in to see
抗原
适用
Discosoma
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宿主

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克隆类型
多克隆
标记
This RFP antibody is conjugated to Biotin
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应用范围
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Supplier
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免疫原 The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Type of Immunogen: Fusion protein
亚型 IgG
特异性 Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1 % of target signal.
纯化方法 Affinity purified
别名 RFP
研究领域 Tags/Labels
应用备注 Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
限制 仅限研究用
状态 Liquid
溶解方式 deionized water
浓度 Lot specific
缓冲液 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 avoid freeze thaw cycles.
储存条件 4 °C,-20 °C
储存方法 Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
厂商提供的图像
 image for anti-RFP 抗体 (Red Fluorescent Protein)  (Biotin) (ABIN1103973) anti-Red Fluorescent Protein (RFP) antibody (Biotin)