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Myc 标记 (AA 410-419) 抗体 (Biotin)

ABIN1103150 产品详细信息, 供应商: Log in to see
抗原
抗原表位
AA 410-419
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10
6
4
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2
2
2
2
2
1
1
适用
标记
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36
14
10
6
5
3
1
1
宿主

161
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3
2
1
克隆类型
多克隆
标记
Biotin
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22
14
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
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1
应用范围
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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22
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19
7
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5
5
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3
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2
1
1
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1
1
1
1
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免疫原 This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Type of Immunogen: Purified protein
亚型 IgG
特异性 This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
纯化方法 Affinity purified
物质类 Tag
研究领域 Tags/Labels
应用备注 Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
限制 仅限研究用
状态 Liquid
溶解方式 deionized water
浓度 Lot specific
缓冲液 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 avoid freeze thaw cycles.
储存条件 4 °C,-20 °C
储存方法 Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
厂商提供的图像
 image for anti-Myc 标记 (AA 410-419) 抗体 (Biotin) (ABIN1103150) anti-Myc Tag (AA 410-419) antibody (Biotin)