His 标记 抗体 (Biotin)

ABIN1102041 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
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宿主

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克隆类型
多克隆
标记
Biotin
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应用范围
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Type of Immunogen: Purified protein
序列 HHHHHH
亚型 IgG
特异性 This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
纯化方法 Affinity purified
物质类 Tag
研究领域 Tags/Labels
应用备注 Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
限制 仅限研究用
状态 Liquid
溶解方式 deionized water
浓度 Lot specific
缓冲液 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 avoid freeze thaw cycles.
储存条件 4 °C,-20 °C
储存方法 Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
厂商提供的图像
 image for anti-His Tag antibody (Biotin) (ABIN1102041) anti-His Tag antibody (Biotin)
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