Immunoblotting: use at 1-5 μg/mL. A band of ~60 kDa is detected. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.
限制
仅限研究用
状态
Liquid
溶解方式
Dilute in PBS or medium that is identical to that used in the assay system.
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
抗原
SLC1A1
(Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))
Excitatory amino acid transporter 3,Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7914198, PubMed:7521911, PubMed:8857541, PubMed:26690923, PubMed:21123949). Can also transport L-cysteine (PubMed:21123949). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:7521911, PubMed:8857541, PubMed:26690923). Mediates Cl(-) flux that is not coupled to amino acid transport, this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:8857541, PubMed:26690923). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:21123949). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity). {UniProtKB:P51906, UniProtKB:P51907, PubMed:21123949, PubMed:26690923, PubMed:7521911, PubMed:7914198, PubMed:8857541}.,Excitatory amino-acid carrier 1 (EAAC1) is a high-affinity Na+-dependent L- glutamate/D,L-aspartate cell membrane transport protein. It is expressed in brain as well as in several non-nervous system tissues. In brain, EAAC1 is the primary neuronal glutamate transporter. It has a polarized distribution in cells and functions mainly perisynaptically to transport glutamate from the extracellular environment. In the kidney it is involved in renal acidic amino acid reabsorption and amino acid metabolism. EAAC1- associated protein GTRAP3-18 may be important in regulating the metabolic function of EAAC1.,Cell membrane, Apical cell membrane, Cell junction, synapse, synaptosome, Early endosome membrane, Late endosome membrane, Recycling endosome membrane,Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1