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ATP7A 抗体 (C-Term)

ATP7A 适用: 人 WB, IHC, IP, IF/ICC 宿主: 小鼠 Monoclonal S60-4 unconjugated
产品编号 ABIN1098198
发货至: 中国
  • 抗原 See all ATP7A 抗体
    ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))
    抗原表位
    • 15
    • 14
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 42-61, C-Term
    适用
    • 50
    • 29
    • 28
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    宿主
    • 36
    • 14
    • 1
    • 1
    小鼠
    克隆类型
    • 38
    • 14
    单克隆
    标记
    • 24
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ATP7A antibody is un-conjugated
    应用范围
    • 42
    • 19
    • 14
    • 14
    • 13
    • 13
    • 13
    • 12
    • 7
    • 4
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)
    原理
    Anti-Copper-Transporting ATPase1 Mouse Monoclonal Antibody
    特异性
    This antibody recognizes human, mouse, and rat Copper-Transporting ATPase1.Immunohistochemistry: use at 1-5 μg/mL. These are recommended concentrations.Enduser should determine optimal concentrations for their
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Purified by Protein G affinity chromatography.
    免疫原
    Synthetic peptide correspond- ing to aa 42-61 (cytoplasmic C-terminus) of human Copper-Transporting ATPase1 (accession no. NP_000043.3).
    克隆位点
    S60-4
    亚型
    IgG2b
    Top Product
    Discover our top product ATP7A Primary Antibody
  • 应用备注
    Immunoblotting: use at 1-2 μg/mL. A band of ~180 kDa is detected.
    限制
    仅限研究用
  • 状态
    Liquid
    溶解方式
    Dilute in PBS or medium that is identical to that used in the assay system.
    浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
  • 抗原
    ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))
    别名
    Copper-Transporting ATPase1 (ATP7A 产品)
    别名
    ATP7A antibody, cal antibody, wu:fc43e01 antibody, zgc:153422 antibody, zgc:158633 antibody, DDBDRAFT_0218568 antibody, DDBDRAFT_0235190 antibody, DDB_0218568 antibody, DDB_0235190 antibody, atpase antibody, Atp7a antibody, kal antibody, atp7a antibody, DSMAX antibody, MK antibody, MNK antibody, SMAX3 antibody, Blo antibody, DXHXS1608e antibody, I14 antibody, Mo antibody, blotchy antibody, br antibody, brindled antibody, mottled antibody, Mnk antibody, ATPase copper transporting alpha antibody, ATPase, Cu++ transporting, alpha polypeptide antibody, P-type ATPase antibody, ATP synthase subunit a antibody, copper-transporting ATPase 1 antibody, ATP7A antibody, atp7a antibody, LOC100049514 antibody, Atp7a antibody, LOC412379 antibody
    背景
    Copper-transporting ATPase 1,ATP-driven copper (Cu(+)) ion pump that plays an important role in intracellular copper ion homeostasis (PubMed:10419525, PubMed:11092760, PubMed:28389643). Within a catalytic cycle, acquires Cu(+) ion from donor protein on the cytoplasmic side of the membrane and delivers it to acceptor protein on the lumenal side. The transfer of Cu(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:10419525, PubMed:19453293, PubMed:19917612, PubMed:31283225, PubMed:28389643). Under physiological conditions, at low cytosolic copper concentration, it is localized at the trans-Golgi network (TGN) where it transfers Cu(+) ions to cuproenzymes of the secretory pathway (PubMed:28389643, PubMed:11092760). Upon elevated cytosolic copper concentrations, it relocalizes to the plasma membrane where it is responsible for the export of excess Cu(+) ions (PubMed:10419525, PubMed:28389643). May play a dual role in neuron function and survival by regulating cooper efflux and neuronal transmission at the synapse as well as by supplying Cu(+) ions to enzymes such as PAM, TYR and SOD3 (PubMed:28389643) (By similarity). In the melanosomes of pigmented cells, provides copper cofactor to TYR to form an active TYR holoenzyme for melanin biosynthesis (By similarity). {UniProtKB:Q64430, PubMed:10419525, PubMed:11092760, PubMed:19453293, PubMed:19917612, PubMed:28389643, PubMed:31283225}.,The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from cells. ATP7A (also known as Copper-Transporting ATPase1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Although ATP7A is not detectable in most normal tissues, it is expressed in many common tumor types. Increased expression of ATP7A renders tumor cells resistant to cisplatin and carboplatin.,Golgi apparatus, trans-Golgi network membrane, Cell membrane, Melanosome membrane, Early endosome membrane, Cell projection, axon, Cell projection, dendrite, Cell junction, synapse, postsynaptic density,EC 7.2.2.8, Copper pump 1, Menkes disease-associated protein
    UniProt
    Q04656
    途径
    Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process
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