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alpha Tubulin 抗体 (AA 427-441) (Biotin)

Name: alpha Tubulin 抗体 (AA 427-441) (Biotin)
Brand: antibodies-online
SKU: ABIN1043919
Availability: OutOfStock

TUBA1 适用: 人, 小鼠, 大鼠, 小鸡, Cow, 山羊, 绵羊 WB, ELISA 宿主: 兔 Polyclonal Biotin

(1 reference)

产品编号 ABIN1043919

发货至: 中国

Datasheet as PDF

抗原 See all alpha Tubulin (TUBA1) 抗体

alpha Tubulin (TUBA1)
抗原表位
16

11

6

6

4

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 427-441
适用
107

72

65

24

18

15

14

12

8

5

5

4

4

4

4

4

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, 小鸡, Cow, 山羊, 绵羊
宿主
61

54

8

2

2

1

1
兔
Compatible Secondaries
克隆类型
71

55

2
多克隆
标记
85

10

6

5

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This alpha Tubulin antibody is conjugated to Biotin
应用范围
102

44

42

42

38

33

27

14

7

6

4

2

1

1

1

1
Western Blotting (WB), ELISA

特异性

Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

产品特性

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.

免疫原

Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.

亚型

IgG

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应用备注

Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.

说明

Gene Name: TUBA1B

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

浓度

0.61 mg/ml

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

有效期

12 months
Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, (1985) (PubMed).
抗原

alpha Tubulin (TUBA1)

别名

Alpha-Tubulin (TUBA1 产品)

别名

Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody

背景

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Synonyms: Loading Control Antibody

途径

Microtubule Dynamics