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beta Catenin 抗体 (C-Term)

CATNB 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 非洲爪蟾 WB, ELISA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1043907
发货至: 中国
  • 抗原 See all beta Catenin (CATNB) 抗体
    beta Catenin (CATNB) (Catenin, beta (CATNB))
    抗原表位
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    C-Term
    适用
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    人, 小鼠, 大鼠, Cow, 斑马鱼, 非洲爪蟾
    宿主
    • 272
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    克隆类型
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    多克隆
    标记
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    This beta Catenin antibody is un-conjugated
    应用范围
    • 206
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    Western Blotting (WB), ELISA
    特异性
    Beta catenin antibody is directed against catenin beta-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
    产品特性
    Beta-catenin 1 (or ß-catenin 1) is a protein that is encoded by the CTNNB1 gene. ß-catenin 1 is a subunit of the cadherin protein complex and has been implicated as an integral component in the Wnt signaling pathway. This pathway plays a key role in the regulation of cellular processes involved in development, differentiation, and adult tissue homeostasis. In the presence of Wnt ligand, ß-catenin 1 is not ubiquitinated and accumulates in the nucleus, where it associates with T-cell factor (TCF) family members to regulate target gene expression in many developmental and adult tissues. Recruitment of b-catenin 1 to Wnt response element (WRE) chromatin converts TCFs from transcriptional repressors to activators. ß-catenin 1 is also involved in the regulation of cell adhesion. It acts as a negative regulator of centrosome cohesion. Aberrant Wnt/ß-catenin signaling is widely implicated in cancer, bone disorders, kidney and intestinal cell disorders and other disease states. ß-catenin 1 is located in the cytoplasm when it is unstabilized or bound to CDH1. Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of ß-catenin 1 is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. It further co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
    过滤
    Sterile filtered
    免疫原
    beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-terminus.
    Immunogen Type: Peptide
    亚型
    IgG
  • 应用备注
    beta catenin antibody has been tested for use in ELISA, western blotting, and ISH. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85.5 kDa in size corresponding to catenin beta-1 protein by western blotting in the appropriate cell lysate or extract.
    说明

    Gene Name: ctnnb1

    限制
    仅限研究用
  • 生效 #029816 (Western Blotting)
    '独立验证'标志
    by
    ADS Biosystems Inc
    No.
    #029816
    日期
    2014.09.18
    抗原
    Lot Number
    27397
    Method validated
    Western Blotting
    Positive Control
    MCF-7 cells
    Negative Control
    SK-BR-3 cells
    Notes
    A strong specific band was observed in the positive control at the expected size (~85.5 kDa) that is not observed in the negative control.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Beta Catenin
    • Catalog number: ABIN1043907
    • Lot number: 27397
    • Dilution: 1:1,000
    Secondary Antibody
    • Antibody: IRDye 680LT Goat Anti-Rabbit
    • Lot number: C30725-01
    • Dilution: 1:10,000
    Full Protocol
    • Lysates were mixed with NuPAGE® LDS Sample Buffer (Life Technologies NP0007) and NuPAGE® Sample Reducing Agent (Life Technologies NP0004) and denatured for 5 minutes at 90ºC.
    • 40 μg of each lysate was electrophoresed on a Bolt 4-12% Bis-Tris Gel (Life Technologies BG04120BOX) and run in Bolt MOPS SDS Running Buffer (Life Technologies B0001) at 160 volts for 1 hour.
    • Odyssey Western Protein Standard (LI-COR #928-40000) was run as a molecular weight standard.
    • PVDF membrane was activated with methanol.
    • Protein samples were transferred to activated PVDF membrane in a wet Bolt Transfer Apparatus (Life Technologies B1000) at room temperature for 1 hour at 20 volts (started at 230mA, ended at 110mA).
    • The membrane was blocked in x LI-COR Odyssey WB block solution for 1 hour at room temperature.
    • The membrane was incubated with the primary antibody diluted 1:1000 in x LI-COR Odyssey WB block solution incubated 2 hours at room temperature.
    • The membrane was washed 4 x 5 minutes in 1 x PBS-T (PBS solution with 0.1% Tween 20).
    • The membrane was incubated with IRDye® 800CW Goat anti-Mouse Secondary Antibody (Red) and IRDye 680LT Goat Anti-Rabbit Secondary Antibody (Green) from LI-COR (#827-11081, Lot #C30725-01), both 1:10,000 dilutions. Incubation was performed at room temperature for 45 minutes.
    • The membrane was washed 4 x 5 minutes in 1 x PBS-T (PBS solution with 0.1% Tween 20).
    • Proteins were detected using Odyssey machine scanning with green channel for loading control and red channel for potential LPL band.
    Experimental Notes
    - No experimental challenges noted.
  • 状态
    Liquid
    浓度
    1.15 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    有效期
    12 months
  • Huge, Sandbothe, Schröder, Stalke, Eilers, Schäffer, Schlegelberger, Illig, Vajen, Skawran: "Wnt status-dependent oncogenic role of BCL9 and BCL9L in hepatocellular carcinoma." in: Hepatology international, Vol. 14, Issue 3, pp. 373-384, (2020) (PubMed).

    Mo, Chew, Maher, Bellipanni, Weinberg, Gottardi: "The terminal region of beta-catenin promotes stability by shielding the Armadillo repeats from the axin-scaffold destruction complex." in: The Journal of biological chemistry, Vol. 284, Issue 41, pp. 28222-31, (2009) (PubMed).

  • 抗原
    beta Catenin (CATNB) (Catenin, beta (CATNB))
    别名
    beta Catenin (CATNB 产品)
    别名
    Bfc antibody, Catnb antibody, Mesc antibody, CTNNB antibody, MRD19 antibody, armadillo antibody, ctnnb antibody, id:ibd2058 antibody, wu:fb73e10 antibody, wu:fi81c06 antibody, wu:fk25h01 antibody, beta-catenin antibody, ctnnb1 antibody, CHBCAT antibody, catenin (cadherin associated protein), beta 1 antibody, catenin beta 1 antibody, catenin (cadherin-associated protein), beta 1 antibody, catenin beta 1 L homeolog antibody, Ctnnb1 antibody, CTNNB1 antibody, ctnnb1 antibody, ctnnb1.L antibody
    背景
    Beta-catenin 1 (or ß-catenin 1) is a protein that is encoded by the CTNNB1 gene. ß-catenin 1 is a subunit of the cadherin protein complex and has been implicated as an integral component in the Wnt signaling pathway. This pathway plays a key role in the regulation of cellular processes involved in development, differentiation, and adult tissue homeostasis. In the presence of Wnt ligand, ß-catenin 1 is not ubiquitinated and accumulates in the nucleus, where it associates with T-cell factor (TCF) family members to regulate target gene expression in many developmental and adult tissues. Recruitment of b-catenin 1 to Wnt response element (WRE) chromatin converts TCFs from transcriptional repressors to activators. ß-catenin 1 is also involved in the regulation of cell adhesion. It acts as a negative regulator of centrosome cohesion. Aberrant Wnt/ß-catenin signaling is widely implicated in cancer, bone disorders, kidney and intestinal cell disorders and other disease states. ß-catenin 1 is located in the cytoplasm when it is unstabilized or bound to CDH1. Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of ß-catenin 1 is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. It further co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
    Synonyms: Catenin beta-1, beta catenin, ß-catenin-1, ß-catenin, CTNNB1, CTNNB, beta catenin antibody, anti-beta catenin
    NCBI登录号
    NP_001091679
    UniProt
    Q9WU82
    途径
    Peptide Hormone Metabolism
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