我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

PDCD4 抗体 (C-Term)

Name: PDCD4 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN1043890
Availability: OutOfStock

PDCD4 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

H2O2 causes cytoplasmic accumulation of HuR and a loss in PDCD4 expression that is mediated by miR-21A.

Western blot using affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead).

Western Blot of Rabbit anti-PDCD antibody.

HuR regulates PDCD4 stability via miR-21A.

HuR directly binds to PDCD4 3'UTR mRNA to regulate its protein expressionA.

ERK8 phosphorylates HuR to prevent its binding to PDCD4 mRNAA.

8 images

(11 references)

产品编号 ABIN1043890

发货至: 中国

Datasheet as PDF

抗原 See all PDCD4 抗体

PDCD4 (Programmed Cell Death 4 (PDCD4))
抗原表位
15

15

13

8

8

7

6

6

6

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
104

70

60

5

4

4

4

3

3

3

3

3

3

1

1
人
宿主
96

6

2

1
兔
Compatible Secondaries
克隆类型
92

13
多克隆
标记
59

7

4

4

4

4

4

4

3

3

2

2

1

1

1

1

1
This PDCD4 antibody is un-conjugated
应用范围
76

45

36

16

14

14

13

9

7

3

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

交叉反应

小鼠, 大鼠, 非洲爪蟾

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the of human Pdcd4 protein.

亚型

IgG
anti-Programmed Cell Death 4 (PDCD4) (C-Term) antibody
PDCD4 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibody
PDCD4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-100) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibody
PDCD4 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal K4C1 unconjugated

anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibody
PDCD4 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibody
PDCD4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (N-Term) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (pSer67) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 230-330) antibody
PDCD4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (Internal Region), (pSer457) antibody (Biotin)
PDCD4 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 Biotin

应用备注

This affinity purified antibody has been tested for use in ELISA, western blotting, immunoprecipitation and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Pdcd4 protein by western blotting in the appropriate cell lysate or extract.

限制

仅限研究用
状态

Liquid

浓度

1.06 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Hill, Nesser, Johnson-Camacho, Jeffress, Johnson, Boniface, Spencer, Lu, Heiser, Lawrence, Pande, Korkola, Gray, Mills, Mukherjee, Spellman: "Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling." in: Cell systems, Vol. 4, Issue 1, pp. 73-83.e10, (2019) (PubMed).

Kim, Hu, Jadhav, Jin, Zhang, Cavanagh, Akondy, Ahmed, Weyand, Goronzy: "Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells." in: Cell reports, Vol. 25, Issue 8, pp. 2148-2162.e5, (2018) (PubMed).

Liwak-Muir, Dobson, Naing, Wylie, Chehade, Baird, Chakraborty, Holcik: "ERK8 is a novel HuR kinase that regulates tumour suppressor PDCD4 through a miR-21 dependent mechanism." in: Oncotarget, Vol. 7, Issue 2, pp. 1439-50, (2016) (PubMed).

Sharma, Lin, Farrugia, McLaughlin, Ellis, Brundage, Salkeni, Ruppert: "MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1." in: Molecular and cellular biology, Vol. 34, Issue 22, pp. 4143-64, (2015) (PubMed).

Yang, Yue, Sims, Pfeffer: "The curcumin analog EF24 targets NF-κB and miRNA-21, and has potent anticancer activity in vitro and in vivo." in: PLoS ONE, Vol. 8, Issue 8, pp. e71130, (2014) (PubMed).

Hirata, Suzuki, Imaeda, Matsuzaki, Tsugawa, Nagano, Asakura, Saya, Hibi: "CD44 variant 9 expression in primary early gastric cancer as a predictive marker for recurrence." in: British journal of cancer, Vol. 109, Issue 2, pp. 379-86, (2013) (PubMed).

Ruan, Wang, Kameswaran, Wei, Johnson, Matschinsky, Shi, Chen: "The microRNA-21-PDCD4 axis prevents type 1 diabetes by blocking pancreatic beta cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 29, pp. 12030-5, (2011) (PubMed).

Santhanam, Baker, Hegamyer, Kirschmann, Colburn: "Pdcd4 repression of lysyl oxidase inhibits hypoxia-induced breast cancer cell invasion." in: Oncogene, Vol. 29, Issue 27, pp. 3921-32, (2010) (PubMed).

OHayre, Salanga, Kipps, Messmer, Dorrestein, Handel: "Elucidating the CXCL12/CXCR4 Signaling Network in Chronic Lymphocytic Leukemia through Phosphoproteomics Analysis." in: PLoS ONE, Vol. 5, Issue 7, pp. e11716, (2010) (PubMed).

Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).
抗原

PDCD4 (Programmed Cell Death 4 (PDCD4))

别名

Pdcd4 (PDCD4 产品)

别名

H731 antibody, D19Ucla1 antibody, Ma3 antibody, Tis antibody, Dug antibody, cb45 antibody, pdcd4 antibody, sb:cb45 antibody, wu:fc84b08 antibody, h731 antibody, MGC69337 antibody, PDCD4 antibody, wu:fa55g03 antibody, wu:fb08a11 antibody, wu:fe01a02 antibody, zgc:66311 antibody, programmed cell death 4 antibody, programmed cell death 4b antibody, programmed cell death 4 L homeolog antibody, programmed cell death 4 (neoplastic transformation inhibitor) antibody, programmed cell death 4a antibody, PDCD4 antibody, Pdcd4 antibody, pdcd4b antibody, pdcd4.L antibody, pdcd4 antibody, pdcd4a antibody

背景

This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.
Synonyms: Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

基因ID

27250, 21735596

UniProt

Q53EL6