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Interleukin 6 抗体 (IL6) (HRP)

Details for Product anti-IL6 Antibody No. ABIN1043799, 供应商: Log in to see
抗原
  • Il-6
  • ILg6
  • Ifnb2
  • BSF2
  • HGF
  • HSF
  • IFNB2
  • IL-6
  • CHIL-6
  • il6
  • interleukin 6
  • interleukin 6 (interferon, beta 2)
  • interleukin-6
  • Il6
  • IL6
  • il-6
Alternatives
anti-人 Interleukin 6 抗体 for Blocking Antibody
适用

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宿主

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克隆类型
多克隆
标记
This Interleukin 6 antibody is conjugated to HRP
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应用范围
Neutralization (Neut), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
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Supplier
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免疫原 This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
Immunogen Type: RecombinantProtein
亚型 IgG
特异性 This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.  A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
产品特性 This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
纯化方法 purified
别名 IL-6 (IL6 Antibody 摘要)
背景 IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Synonyms: HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
基因ID 3569
NCBI登录号 NP_000591
UniProt P05231
研究领域 Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors
途径 Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis
应用备注 This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
浓度 1.1 mg/ml
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存液 Gentamicin sulfate
注意事项 Do NOT add Sodium Azide!
储存条件 4 °C/-20 °C
储存方法 Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-Interleukin 6 抗体 (IL6)  (HRP) (ABIN1043799) Western Blot of Peroxidase conjugated Rabbit anti-Human IL-6 antibody. Lane 1: Human ...
有引用在: Novick: "Current concepts in the management of renovascular hypertension and ischemic renal failure." in: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 13, Issue 6 Suppl 1, pp. 33-7, 1989 (PubMed).