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AKT1 抗体 (pThr308)

Name: AKT1 抗体 (pThr308)
Brand: antibodies-online
SKU: ABIN1043729
Availability: OutOfStock

AKT1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IP 宿主: 小鼠 Monoclonal 18F3-H11 unconjugated

Western Blot of Mouse anti-AKT pT308 antibody.

Immunohistochemistry of Mouse anti-AKT pT308 antibody.

IHC

2 images

(4 references)

产品编号 ABIN1043729

发货至: 中国

Datasheet as PDF

抗原 See all AKT1 抗体

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
抗原表位
93

45

36

33

32

21

20

19

19

17

16

11

11

9

9

9

8

8

8

8

8

8

8

7

7

5

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2
pThr308
适用
543

352

280

55

48

46

34

19

12

12

4

4

3

3

2

2

1

1

1

1
人, 小鼠, 大鼠, 猴
宿主
477

86

7

2

1
小鼠
Compatible Secondaries
克隆类型
432

140
单克隆
标记
308

39

28

25

22

19

14

14

14

13

12

12

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1
This AKT1 antibody is un-conjugated
应用范围
441

257

189

84

82

73

50

50

31

29

28

15

12

9

9

4

3

3

3

2

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

特异性

Anti-AKT pT308 antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur.

产品特性

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.

过滤

Sterile filtered

免疫原

Akt phospho T308 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Immunogen Type: Peptide

克隆位点

18F3-H11

亚型

IgG1 kappa

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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
AKT1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2E11 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
AKT1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
AKT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody
AKT1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
AKT1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IP, FM, IHC (fp) 宿主: 兔 Polyclonal unconjugated

应用备注

Anti-AKT Phopsho T308 monoclonal antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

说明

Gene Name: AKT1

限制

仅限研究用
状态

Liquid

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.

有效期

12 months
Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, (2001) (PubMed).

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, (2001) (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, (1991) (PubMed).

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, (1987) (PubMed).
抗原

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

别名

AKT (AKT1 产品)

别名

AKT antibody, CWS6 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, Akt antibody, Rac antibody, PKB/Akt antibody, PKBalpha antibody, AKT/PKB antibody, AKT1 antibody, Akt/PKB antibody, Akt1 antibody, CG4006 antibody, D-Akt antibody, DAKT1 antibody, DAKT1/PKB antibody, DAkt antibody, DAkt1 antibody, DPKB antibody, DRAC-PK antibody, DRAC-PK66 antibody, DRAC-PK85 antibody, Dakt antibody, Dakt1 antibody, Dmel\\CG4006 antibody, Dpkb antibody, PKB/AKT antibody, PKB/dAKT antibody, RacPK antibody, akt antibody, akt1 antibody, dAKT antibody, dAKT/dPKB antibody, dAKT1 antibody, dAkt antibody, dAkt/PKB antibody, dAkt1 antibody, dPKB antibody, dakt antibody, dakt1 antibody, l(3)04226 antibody, l(3)89Bq antibody, p-Akt antibody, pAkt antibody, ACT-5 antibody, akt-1 antibody, pkb antibody, v-akt antibody, v-akt1 antibody, xAct antibody, ATAKT1 antibody, F18A8.2 antibody, F18A8_2 antibody, K+ transporter 1 antibody, POTASSIUM TRANSPORTER antibody, AKT serine/threonine kinase 1 antibody, thymoma viral proto-oncogene 1 antibody, CG4006 gene product from transcript CG4006-RE antibody, actin beta antibody, v-akt murine thymoma viral oncogene homolog 1 S homeolog antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, K+ transporter 1 antibody, Serine/threonine-protein kinase akt-1 antibody, AKT1 antibody, Akt1 antibody, ACTB antibody, akt1.S antibody, akt1 antibody, KT1 antibody, akt-1 antibody

背景

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

基因ID

207, 62241011

UniProt

P31749

途径

PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect