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KLRK1 抗体 (FITC)

Name: KLRK1 抗体 (FITC)
Brand: antibodies-online
SKU: ABIN1027688
Availability: OutOfStock

KLRK1 适用: 人 FACS 宿主: 小鼠 Monoclonal 1D11 FITC

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 μL reagent / 100 μL of peripheral whole blood).

FACS

Surface staining of human peripheral blood with anti-human CD314 (1D11) FITC.

FACS

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD314 (1D11) FITC antibody (4 μL reagent / 100 μL of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 μL reagent / 100 μL of peripheral whole blood).

FACS

Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 μL reagent / 100 μL of peripheral whole blood).

FACS

4 images

(7 references)

产品编号 ABIN1027688

发货至: 中国

Datasheet as PDF

抗原 See all KLRK1 抗体

KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
适用
54

32

8

6

4

3

1

1

1

1
人
宿主
55

16

3

2

2

1
小鼠
Compatible Secondaries
克隆类型
54

23

2
单克隆
标记
35

8

5

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This KLRK1 antibody is conjugated to FITC
应用范围
44

43

15

14

14

13

11

11

7

4

4

4

4

2

2

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody 1D11 recognizes an extracellular epitope of CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

NKL cell line

克隆位点

1D11

亚型

IgG1 kappa

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anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 121-219) antibody
KLRK1 适用: 小鼠 WB, FACS, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody
KLRK1 适用: 人 FACS, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal 1D11 unconjugated

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody
KLRK1 适用: 人 FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal 1D11 unconjugated

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 167-216) antibody
KLRK1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 73-216) antibody
KLRK1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 92-216) antibody
KLRK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 90-232) antibody
KLRK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 75-215) antibody
KLRK1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (Internal Region) antibody
Verified KLRK1 适用: 人 WB, FACS, ELISA 宿主: 山羊 Polyclonal unconjugated

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Valencia, Hernández-López, Martínez, Hidalgo, Zapata, Vicente, Varas, Sacedón: "Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors." in: Journal of leukocyte biology, Vol. 87, Issue 3, pp. 405-14, (2010) (PubMed).

Hasenkamp, Borgerding, Uhrberg, Falk, Chapuy, Wulf, Jung, Trümper, Glass: "Self-tolerance of human natural killer cells lacking self-HLA-specific inhibitory receptors." in: Scandinavian journal of immunology, Vol. 67, Issue 3, pp. 218-29, (2008) (PubMed).

Sangiolo, Martinuzzi, Todorovic, Vitaggio, Vallario, Jordaney, Carnevale-Schianca, Capaldi, Geuna, Casorzo, Nash, Aglietta, Cignetti: "Alloreactivity and anti-tumor activity segregate within two distinct subsets of cytokine-induced killer (CIK) cells: implications for their infusion across major HLA barriers." in: International immunology, Vol. 20, Issue 7, pp. 841-8, (2008) (PubMed).

Ebert, Meuter, Moser: "Homing and function of human skin gammadelta T cells and NK cells: relevance for tumor surveillance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 7, pp. 4331-6, (2006) (PubMed).

Wu, Groh, Spies: "T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 3, pp. 1236-40, (2002) (PubMed).

Wu, Cherwinski, Spies, Phillips, Lanier: "DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells." in: The Journal of experimental medicine, Vol. 192, Issue 7, pp. 1059-68, (2000) (PubMed).

Bauer, Groh, Wu, Steinle, Phillips, Lanier, Spies: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, (1999) (PubMed).
抗原

KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

别名

CD314 (KLRK1 产品)

别名

KLRK1 antibody, NKG2D antibody, CD314 antibody, D12S2489E antibody, KLR antibody, NKG2-D antibody, D6H12S2489E antibody, Nkg2d antibody, Nkrp2 antibody, killer cell lectin like receptor K1 antibody, killer cell lectin-like receptor subfamily K, member 1 antibody, NKG2D protein antibody, KLRK1 antibody, Klrk1 antibody, NKG2D antibody

背景

Killer cell lectin like receptor K1,CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.,NKG2D, KLRK1, KLR

基因ID

22914

UniProt

P26718

途径

Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process