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兔 anti-小鼠 IgG3 (Heavy Chain) Antibody (Biotin) - Preadsorbed

ELISA, WB, IHC Polyclonal IgG Biotin
产品编号 ABIN102435
发货至: 中国
  • 抗原 See all IgG3 products
    IgG3
    抗原表位
    • 37
    • 15
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    Heavy Chain
    适用
    • 70
    • 19
    • 3
    小鼠
    宿主
    • 37
    • 27
    • 17
    • 10
    • 1
    克隆类型
    • 63
    • 27
    多克隆
    标记
    • 18
    • 12
    • 10
    • 10
    • 6
    • 5
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    应用范围
    • 50
    • 39
    • 37
    • 32
    • 25
    • 21
    • 12
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    特异性
    IgG3
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG3 heavy chain

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

    ELISA Dilution: 1:8,000 - 1:32,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG3
    Abstract
    IgG3 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit Anti-Mouse IgG3 (Gamma 3 chain) Biotin Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody Biotin Conjugation

    Background: Anti-Mouse IgG3 Biotin Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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