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兔 anti-小鼠 IgG2b (Heavy Chain) Antibody (TRITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG TRITC
产品编号 ABIN102406
发货至: 中国
  • 抗原 See all IgG2b products
    IgG2b
    抗原表位
    • 35
    • 16
    • 5
    • 3
    Heavy Chain
    适用
    • 80
    • 24
    小鼠
    宿主
    • 60
    • 24
    • 12
    • 7
    • 1
    克隆类型
    • 71
    • 19
    多克隆
    标记
    • 17
    • 11
    • 10
    • 8
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    TRITC
    应用范围
    • 48
    • 44
    • 33
    • 29
    • 27
    • 20
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    IgG2b
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG2b heavy chain

    亚型
    IgG
    Labeling Ratio
    3.0
  • 应用备注

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG2b
    Abstract
    IgG2b 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody rhodamine Conjugated, Rabbit Anti-Mouse IgG2b Antibody TRITC Conjugation

    Background: Anti-Mouse IgG2b Rhodamine Antibody generated in rabbit detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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