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兔 anti-小鼠 IgG1 (Heavy Chain) Antibody (Biotin) - Preadsorbed

ELISA, WB, IHC Polyclonal IgG Biotin
产品编号 ABIN102335
发货至: 中国
  • 抗原 See all IgG1 products
    IgG1
    抗原表位
    • 41
    • 26
    • 17
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain
    适用
    • 84
    • 25
    • 23
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    小鼠
    宿主
    • 64
    • 39
    • 34
    • 10
    • 9
    • 6
    • 1
    克隆类型
    • 96
    • 54
    多克隆
    标记
    • 44
    • 18
    • 18
    • 15
    • 11
    • 8
    • 7
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    应用范围
    • 75
    • 56
    • 51
    • 41
    • 32
    • 21
    • 18
    • 12
    • 11
    • 10
    • 10
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    特异性
    IgG1
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG1 heavy chain

    亚型
    IgG
    Labeling Ratio
    10-20
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:3,000

    Application Note: Mouse IgG1 secondary antibody conjugated to Biotin is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

    ELISA Dilution: 1:250,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG1
    Abstract
    IgG1 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody biotin Conjugation, Rabbit Anti-Mouse IgG1 BAC Conjugated Antibody

    Background: Anti-Mouse IgG1 Biotin Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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