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兔 anti-大鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
产品编号 ABIN102162
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Heavy & Light Chain
    适用
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    大鼠
    宿主
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    克隆类型
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    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG (H&L)
    交叉反应
    大鼠
    产品特性
    Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically rat IgG (H&L). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in rabbit

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000-1:5,000

    Application Note: Antibody anti-Rat has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Antibody anti-Rat has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:20,000 - 1:300,000

    Western Blot Dilution: 1:2,000-1:10,000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2.2 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti-Rat IgG (H&L) Antibody, Rabbit-a-Rat IgG (H&L), Rat IgG Antibody in rabbit, anti-Rat IgG Secondary Antibody

    Background: Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically rat IgG (H&L). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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