驴 anti-兔 IgG (Heavy & Light Chain) Antibody (HRP)

ABIN101955 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Heavy & Light Chain
5027
2578
1931
1069
1007
857
330
299
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
适用

2912
2859
2404
1752
1485
796
623
442
381
380
355
340
307
293
208
148
139
114
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
宿主

6893
4912
1877
895
703
401
92
54
52
21
9
8
5
5
2
1
1
克隆类型
多克隆
标记
HRP
2234
1889
1740
1374
765
679
519
300
299
288
274
268
217
188
178
124
103
79
69
60
54
46
43
40
40
36
34
32
29
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
16
15
15
15
15
15
14
12
12
11
10
10
9
9
9
9
9
9
9
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
应用范围
Dot Blot (DB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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免疫原 Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
亚型 IgG
特异性 IgG (H&L)
产品特性 Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
纯化方法 This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
背景 Synonyms: Rabbit Antibody peroxidase conjugated, Dk-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in donkey, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
研究领域 Immunology, Secondary Antibodies
应用备注 Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
限制 仅限研究用
状态 Lyophilized
溶解方式 Restore with deionized water (or equivalent)
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Gentamicin sulfate
注意事项 Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
厂商提供的图像
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit...
背景 Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).