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山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Texas Red (TR)
产品编号 ABIN101770
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
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    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
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    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 691
    • 672
    • 559
    • 452
    • 366
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    • 147
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    • 131
    • 122
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    小鼠
    宿主
    • 1717
    • 1474
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    山羊
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
    • 556
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    • 10
    Texas Red (TR)
    应用范围
    • 1837
    • 1789
    • 1786
    • 1499
    • 962
    • 933
    • 912
    • 515
    • 234
    • 115
    • 115
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    • 100
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    • 4
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    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    IgG (H&L)
    产品特性
    Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    3.9
  • 应用备注

    Application Note: Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    说明

    Texas Red™ is a registered trademark of Molecular Probes Inc.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • Chaillou, Zhang, McCarthy: "Expression of Muscle-Specific Ribosomal Protein L3-Like Impairs Myotube Growth." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1894-902, (2017) (PubMed).

    Fry, Lee, Mula, Kirby, Jackson, Liu, Yang, Mendias, Dupont-Versteegden, McCarthy, Peterson: "Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia." in: Nature medicine, Vol. 21, Issue 1, pp. 76-80, (2015) (PubMed).

    Liu, Fry, Mula, Jackson, Lee, Peterson, Yang: "Automated fiber-type-specific cross-sectional area assessment and myonuclei counting in skeletal muscle." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 115, Issue 11, pp. 1714-24, (2014) (PubMed).

    Fry, Lee, Jackson, Kirby, Stasko, Liu, Dupont-Versteegden, McCarthy, Peterson: "Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 4, pp. 1654-65, (2014) (PubMed).

    Mula, Lee, Liu, Yang, Peterson: "Automated image analysis of skeletal muscle fiber cross-sectional area." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 114, Issue 1, pp. 148-55, (2013) (PubMed).

    McCarthy, Mula, Miyazaki, Erfani, Garrison, Farooqui, Srikuea, Lawson, Grimes, Keller, Van Zant, Campbell, Esser, Dupont-Versteegden, Peterson: "Effective fiber hypertrophy in satellite cell-depleted skeletal muscle." in: Development (Cambridge, England), Vol. 138, Issue 17, pp. 3657-66, (2011) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Goat Anti Mouse IgG (H&L) Texas Red™ Conjugated Antibody Pre-Adsorbed, Goat Anti-Mouse IgG Antibody Texas Red™ Conjugation

    Background: Anti-Mouse IgG Texas Red Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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