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山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

FACS, FM Polyclonal IgG FITC
产品编号 ABIN101753
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
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    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 691
    • 672
    • 559
    • 452
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    小鼠
    宿主
    • 1717
    • 1474
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    山羊
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
    • 556
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    • 10
    FITC
    应用范围
    • 1837
    • 1789
    • 1786
    • 1499
    • 962
    • 933
    • 913
    • 515
    • 234
    • 115
    • 115
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    特异性
    IgG (H&L)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    6.6
  • 应用备注

    Application Note: Mouse secondary antibody conjugated to FITC is available in a variety of formats. Anti IgG secondary antibody conjugated is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti mouse IgG FITC is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Flow Cytometry Dilution: 1:1,000 - 1:5,000

    IF Microscopy Dilution: 1:500 - 1:2,500

    说明

    Excitation/Emission wavelength: 494 nm/514 nm

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Goat Anti-Mouse IgG Secondary Antibody fluorescein Conjugated, Goat Anti-Mouse IgG Antibody FITC Conjugated, GAM-FITC, Anti-mouse IgG secondary antibody, anti-mouse IgG Fluorescein conjugated secondary antibody

    Background: Anti-Mouse IgG Fluorescein Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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