Anti-Mouse (H&L) peroxidase conjugated antibody generated in chicken detects specifically mouse IgG (H&L). This secondary peroxidase conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
免疫原
Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in chicken.
Antibody Anti-Mouse IgG (H&L) peroxidase conjugated has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with deionized water (or equivalent)
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液
Gentamicin sulfate
注意事项
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.