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山羊 anti-人 IgG (Heavy & Light Chain) Antibody

ELISA, IHC, WB Polyclonal unconjugated
产品编号 ABIN101508
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
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    Heavy & Light Chain
    适用
    • 692
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    宿主
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    山羊
    克隆类型
    • 3346
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    • 1
    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG (H&L)
    产品特性
    Concentration Definition: by Refractometry
    纯化方法
    This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Human IgG and Human Serum.
    免疫原

    Immunogen: Human IgG whole molecule

  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    90 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: None

    储存液
    Without preservative
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Human IgG Antibody, goat anti Human IgG

    Background: Anti-Human IgG (H&L) generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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