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兔 anti-马 IgG (F(ab')2 Region) Antibody (FITC)

FACS, FLISA, FM Polyclonal IgG FITC
产品编号 ABIN101438
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2333
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    • 410
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    • 15
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    • 6
    • 6
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    F(ab')2 Region
    适用
    • 692
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    • 2
    • 1
    • 1
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    • 1
    宿主
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    • 33
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    克隆类型
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    • 1
    多克隆
    标记
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    FITC
    应用范围
    • 1837
    • 1789
    • 1786
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    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    IgG F(ab')2
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum.  No reaction was observed against Horse IgG F(c).
    免疫原

    Immunogen: Horse IgG F(ab')2 fragment

    亚型
    IgG
    Labeling Ratio
    2.4
  • 应用备注

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    说明

    Excitation/Emission wavelength: 494 nm/514 nm

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Thimerosal

    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti-Horse IgG Fab2 Fluorescein Conjugated Antibody, rabbit anti-Horse IgG Fab2 fragment FITC Conjugated Antibody, rabbit anti-Horse IgG F(ab')2 Antibody Fluorescein Conjugated, rabbit anti-Horse IgG F(ab')2 Antibody FITC Conjugated

    Background: Anti-Horse IgG F(ab')2 Fluorescein Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind horse IgG F(c) will not bind horse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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