兔 anti-豚鼠 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
-
- 抗原 See all IgG products
- IgG
-
抗原表位
- Fc Region
-
适用
- 豚鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- Alkaline Phosphatase (AP)
-
应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG F(c)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: Guinea Pig IgG F(c) fragment
- 亚型
- IgG
-
-
- 应用备注
-
Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Guinea Pig IgG F(c) Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:5,000 - 1:15,000
Western Blot Dilution: 1:500 - 1:2,500
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
-
Synonyms: Rabbit Anti-Guinea Pig IgG F(c) Antibody Alkaline Phosphatase Conjugated, Rabbit Anti-Guinea Pig IgG Fc Fragment Antibody alk phos Conjugated
Background: Anti-Guinea Pig IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of guinea pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
-