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兔 anti-豚鼠 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
产品编号 ABIN101334
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2333
    • 498
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Fc Region
    适用
    • 692
    • 672
    • 559
    • 452
    • 366
    • 238
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    豚鼠
    宿主
    • 1718
    • 1473
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
    • 556
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    • 367
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    • 120
    • 99
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    • 21
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    • 10
    Alkaline Phosphatase (AP)
    应用范围
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
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    • 8
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    • 4
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG F(c)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads
    过滤
    Sterile filtered
    免疫原

    Immunogen: Guinea Pig IgG F(c) fragment

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Anti-Guinea Pig IgG F(c) Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:5,000 - 1:15,000

    Western Blot Dilution: 1:500 - 1:2,500

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit Anti-Guinea Pig IgG F(c) Antibody Alkaline Phosphatase Conjugated, Rabbit Anti-Guinea Pig IgG Fc Fragment Antibody alk phos Conjugated

    Background: Anti-Guinea Pig IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of guinea pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

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