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兔 anti-山羊 IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
产品编号 ABIN101237
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2333
    • 499
    • 410
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    F(ab')2 Region
    适用
    • 692
    • 672
    • 559
    • 452
    • 365
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    山羊
    宿主
    • 1718
    • 1473
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
    • 556
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    • 367
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    • 120
    • 99
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    • 10
    Alkaline Phosphatase (AP)
    应用范围
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
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    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG F(ab')2
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Goat IgG F(ab')2 fragment

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: This product has been assayed against 1.0 μg of Goat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 is suggested for this product.

    ELISA Dilution: 1:2,000 - 1:10,000

    Western Blot Dilution: 1:500 - 1:2,500

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti-goat IgG F(ab')2 ALP conjugated, rabbit anti-goat IgG F(ab')2 alkaline phosphatase conjugated, rabbit anti-goat IgG Fab2 ALP conjugated, rabbit anti-goat IgG Fab2 alkaline phosphatase conjugated

    Background: Anti-Goat IgG F(ab')2 Alkaline Phosphatase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.

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