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驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
产品编号 ABIN101180
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
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    • 1
    Heavy & Light Chain
    适用
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    山羊
    宿主
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    克隆类型
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    • 1
    多克隆
    标记
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    Biotin
    应用范围
    • 1836
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG (H&L)
    交叉反应
    山羊
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey.

    Immunogen Type: Native Protein

    亚型
    IgG
    Labeling Ratio
    10-20
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Goat IgG (H&L) Antibody is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

    ELISA Dilution: 1:600,000

    Western Blot Dilution: 1:60,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • Yamashita, Ohmoto, Yamaguchi, Matsumoto, Hirota: "Skn-1a/Pou2f3 functions as a master regulator to generate Trpm5-expressing chemosensory cells in mice." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189340, (2018) (PubMed).

    He, Qiu, Dai, Fang, Sun, Gong, Ding, Sun: "MicroRNA-351 inhibits denervation-induced muscle atrophy by targeting TRAF6." in: Experimental and therapeutic medicine, Vol. 12, Issue 6, pp. 4029-4034, (2016) (PubMed).

    Tsoulis, Chang, Moore, Chan, Gohir, Petrik, Vickers, Connor, Sloboda: "Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring." in: Biology of reproduction, Vol. 94, Issue 4, pp. 94, (2016) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: donkey anti-Goat IgG Antibody biotin Conjugation, donkey anti-Goat IgG biotin Conjugated Antibody

    Background: Anti-Goat IgG Biotin Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Goat IgG (H&L) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology research.

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