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I kappa B Kinase Detection Set

产品编号 ABIN2456013
发货至: 中国
  • 宿主
    克隆类型
    多克隆
    应用范围
    Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
    纯化方法
    Antibodies are supplied as affinity chromatography purified IgG.
    组件
    IKK±, Antibody, (50 μg)
    IKK², Antibody, (50 μg)
    IKK³ Antibody, (50 μg)
    IKK? Antibody, (50 μg)
  • 应用备注
    These polyclonal antibodies can be used for detection of IKK±, IKK², IKK³ and IKK? by immunoblot at 0.5 - 2 μg/mL, and by immuncytochemistry (IKK±, IKK², and IKK³) or immunohistochemistry (IKK?) at 1 - 10 μg/mL, and Immunoflourescence.
    Optimal dilutions for each application to be determined by the researcher.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS containing 0.02 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Stable at 4 °C for three months, store at -20 °C for up to one year.
    有效期
    12 months
  • 背景
    Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB kinase (IKK), which phosphorylates IκB and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα, and IKKβ,). IKKα, and IKKβ, interact with each other and both are essential for the NF-κB activation. IKKα, specifically phosphorylates IκB-α, while IKKβ, phosphorylates both IκB-α, and IκB-β,. Another molecule in the IKK complex termed IKKγ (also known as NEMO) interacts with IKKβ, and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. IKKγ was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation. IKKε is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα, and IL-1. IKKε message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.
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