Loading Control Detection Set Alpha-Tubulin, GAPDH
-
- 适用
- 人, 小鼠, 大鼠
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Immunofluorescence (IF), Western Blotting (WB)
- 特异性
- The Alpha-Tubulin and GAPDH antibodies are human, mouse, and rat reactive. These antibodies are expected to also recognize chimpanzee, cow, dog, chicken, and zebrafish proteins. The Alpha-Tubulin antibody is also expected to recognize Xenopus and Drosophila proteins.
- 纯化方法
- Antibodies are supplied as affinity chromatography purified IgG.
- 组件
-
Alpha-Tubulin Antibody
GAPDH Antibody (CT),
-
-
- 应用备注
-
These polyclonal antibodies can be used for detection of Alpha-Tubulin and GAPDH by immunoblot at 0.5-2 μg/mL and Immunoflourescence.
Optimal dilutions for each application to be determined by the researcher. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Stable at 4 °C for three months, store at -20 °C for up to one year.
- 有效期
- 12 months
-
- 背景
- Loading controls are used in immunoblots to normalize the levels of protein detected by confirming that protein loading is the same across the gel. The expression levels of the loading control should not vary between the different sample lanes. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample. This ensures constant expression levels. Thus housekeeping genes such as Beta-actin, Alpha-tubulin, and GAPDH are frequently chosen for use as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Globular tubulin subunits made up of alpha- and beta-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism.
-
You are here:
