Phospho Btk Flex Set (Bead D5)

ABIN1379714 产品详细信息, 供应商: Log in to see
蛋白/底物
  • BTK
  • atk
  • bpk
  • xla
  • imd1
  • agmx1
  • psctk1
  • AGMX1
  • AT
  • ATK
  • BPK
  • IMD1
  • PSCTK1
  • XLA
  • AI528679
  • xid
  • Bruton agammaglobulinemia tyrosine kinase
  • BTK
  • btk
  • Btk
抗原表位
pTyr551
适用

应用范围
Immunoassay (IA), Flow Cytometry (FACS)
选项
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品牌 BD™ Cytometric Bead Array,BD™
产品特性 The BD™ CBA Phospho Btk (Y551) Flex Set is a bead-based immunoassay capable of measuring human Bruton's tyrosine kinase (Btk), a non-receptor tyrosine kinase, which has been tyrosine-phosphorylated at Tyr-551 in denatured cell lysate samples. Human reactivity was determined by testing cell lysates with the BD CBA Phospho Btk (Y551) Flex Set. There are several publications evaluating the biology and function of Btk. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
The Phospho Btk (Y551) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 ml microfuge tube for reconstitution. Reconstitute the standard with 0.1 ml Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/ml and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use
组件 Capture beads, detection reagent, standards
别名 Btk
说明

Bead Position: D5

限制 仅限研究用
状态 Liquid
溶解方式 Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
缓冲液 Aqueous buffered solution containing fetal bovine serum and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho Btk (Y551) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
厂商提供的图像
ELISA image for Phospho Btk Flex Set (Bead D5) (ABIN1379714) Phospho Btk Flex Set (Bead D5)
有引用在: Rawlings, Scharenberg, Park, Wahl, Lin, Kato, Fluckiger, Witte, Kinet: "Activation of BTK by a phosphorylation mechanism initiated by SRC family kinases." in: Science (New York, N.Y.), Vol. 271, Issue 5250, pp. 822-5, 1996 (PubMed).