(2 references)-
- 抗原 See all MAPK14 products
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
- 抗原表位
- pThr180, pTyr182
- 适用
- 人, 小鼠, 大鼠
- 应用范围
- Immunoassay (IA), Flow Cytometry (FACS)
- 品牌
- BD™ Cytometric Bead Array,BD™
- 产品特性
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The BD™ CBA Phospho p38 (T180/Y182) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat p38, a member of the mitogen-activated protein kinase (MAPK) family of kinases, that has been threonine-phosphorylated at Thr-180 and tyrosine-phosphorylated at Tyr-182 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the BD CBA Phospho p38 (T180/Y182) Flex Set. Reactivity with rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho p38 (T180/Y182) Flex Set. The biology and function of p38 has been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 ml microfuge tube for reconstitution. Reconstitute the standard with 0.1 ml Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/ml and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use. - 组件
- Capture beads, detection reagent, standards
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- 说明
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Bead Position: B6
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
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- 抗原
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
- 别名
- p38 (MAPK14 产品)
- 别名
- CSBP Accessory Reagents, CSBP1 Accessory Reagents, CSBP2 Accessory Reagents, CSPB1 Accessory Reagents, EXIP Accessory Reagents, Mxi2 Accessory Reagents, PRKM14 Accessory Reagents, PRKM15 Accessory Reagents, RK Accessory Reagents, SAPK2A Accessory Reagents, p38 Accessory Reagents, p38ALPHA Accessory Reagents, CRK1 Accessory Reagents, Csbp1 Accessory Reagents, Csbp2 Accessory Reagents, Exip Accessory Reagents, Hog Accessory Reagents, Prkm14 Accessory Reagents, Prkm15 Accessory Reagents, Sapk2A Accessory Reagents, p38Hog Accessory Reagents, p38alpha Accessory Reagents, p38b Accessory Reagents, zp38b Accessory Reagents, MAPK14 Accessory Reagents, 186F5S Accessory Reagents, BG:DS00797.3 Accessory Reagents, CG7393 Accessory Reagents, D-p38 Accessory Reagents, D-p38 MAPK Accessory Reagents, D-p38b Accessory Reagents, Dm p38b Accessory Reagents, Dmel\\CG7393 Accessory Reagents, Dmp38b Accessory Reagents, Dp38 Accessory Reagents, Dp38b Accessory Reagents, ESTS:186F5S Accessory Reagents, Mpk34C Accessory Reagents, anon-sts23 Accessory Reagents, dp38b Accessory Reagents, p38 MAPK Accessory Reagents, p38 beta Accessory Reagents, p38B Accessory Reagents, p38Kb Accessory Reagents, p38beta Accessory Reagents, Crk1 Accessory Reagents, p38-alpha Accessory Reagents, p38MAPK Accessory Reagents, p38a Accessory Reagents, csbp Accessory Reagents, mapk14a Accessory Reagents, mxi2 Accessory Reagents, sapk2 Accessory Reagents, sapk2a Accessory Reagents, AP22.98 Accessory Reagents, AP22_98 Accessory Reagents, ATMPK14 Accessory Reagents, mitogen-activated protein kinase 14 Accessory Reagents, SAPK2a Accessory Reagents, MAP kinase 14A Accessory Reagents, MAP kinase p38a Accessory Reagents, MAPK 14A Accessory Reagents, fk28c03 Accessory Reagents, hm:zeh1243 Accessory Reagents, wu:fk28c03 Accessory Reagents, zp38a Accessory Reagents, P38C-CRK Accessory Reagents, mitogen-activated protein kinase 14 Accessory Reagents, mitogen activated protein kinase 14 Accessory Reagents, mitogen-activated protein kinase 14b Accessory Reagents, p38b MAP kinase Accessory Reagents, mitogen-activated protein kinase 14 S homeolog Accessory Reagents, mitogen-activated protein kinase 14a Accessory Reagents, CRK proto-oncogene, adaptor protein Accessory Reagents, MAPK14 Accessory Reagents, Mapk14 Accessory Reagents, mapk14b Accessory Reagents, p38b Accessory Reagents, mapk14.S Accessory Reagents, MPK14 Accessory Reagents, mapk14a Accessory Reagents, CRK Accessory Reagents
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