rlberiotoxin (IbTx)

ABIN1001668 产品详细信息, 供应商: Log in to see
适用
Scorpion
宿主
大肠杆菌(E. Coli)
选项
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序列 QFTDVDCSVS KECWSVCKDL FGVDRGKCMG KKCRCYQ.
特异性 Iberiotoxin is a potent selective blocker of the high conductance Ca2+-activated K+ channels (maxi-K).
产品特性 Source: Mesobuthus tamulus (Eastern Indian scorpion).
Molecular formula: C179H277N51O55S7.
Disulfide bonds between Cys7-Cys28, Cys13-Cys33, and Cys17-Cys35.
纯度 > 98 %
背景 Inhibition of BKCa channels heterologously expressed in HEK293 cells by Iberiotoxin. mSlo channel currents, before and during application of 100nM rIberiotoxin. The mSlo currents were activated by a 50 ms voltage step to +100 mV from a prepulse of -180 mV with intracellular 10 M Ca2+.
分子量 4248
应用备注 50-100 nM
说明

K+ channel toxin -KTx 1.3

限制 仅限研究用
状态 Lyophilized
溶解方式 Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50 g in 1.175ml will provide a stock solution of 10 M.
注意事项 Soluble in Water.
厂商提供的图像
 image for rlberiotoxin (IbTx) (ABIN1001668) mSlo channel currents, before and during application of 100nM rIberiotoxin. The mSlo ...
背景 Meera, Wallner, Toro: "A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 10, pp. 5562-7, 2000 (PubMed).

有引用在: Koschak, Koch, Liu, Kaczorowski, Reinhart, Garcia, Knaus: "[125I]Iberiotoxin-D19Y/Y36F, the first selective, high specific activity radioligand for high-conductance calcium-activated potassium channels." in: Biochemistry, Vol. 36, Issue 7, pp. 1943-52, 1997 (PubMed).

Galvez, Gimenez-Gallego, Reuben, Roy-Contancin, Feigenbaum, Kaczorowski, Garcia: "Purification and characterization of a unique, potent, peptidyl probe for the high conductance calcium-activated potassium channel from venom of the scorpion Buthus tamulus." in: The Journal of biological chemistry, Vol. 265, Issue 19, pp. 11083-90, 1990 (PubMed).