rCharybdotoxin

ABIN1001667 产品详细信息, 供应商: Log in to see
适用
Scorpion
宿主
大肠杆菌(E. Coli)
选项
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序列 QFTNVSCTTS KECWSVCQRL HNTSRGKCMN KKCRCYS.
特异性 Charybdotoxin is a potent selective inhibitor of high conductance (maxi-K), different medium and small conductance Ca2+-activated K+ channels, as well as a voltage-dependent K+ channel (Kv1.3).
产品特性 Source: Leiurus quinquestriatus hebraeus (Yellow scorpion).
Molecular formula: C176H280N58O55S7.
Disulfide bonds between Cys7-Cys28, Cys13-Cys33, and Cys17-Cys35.
纯度 > 98 %
背景 Inhibition of mSlo channels heterologously expressed in HEK293 cells by Charybdotoxin. mSlo channel currents, before and during application of 100nM rCharybdotoxin. The currents were activated by a 50 ms voltage step to +100 mV from a prepulse of -180 mV with intracellular 10M Ca2+.
分子量 4313
应用备注 10-100 nM
说明

K+ channel toxin -KTx 1.1, ChTX, ChTX-Lq1, ChTx-a.

限制 仅限研究用
状态 Lyophilized
溶解方式 Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.160ml will provide a stock solution of 10 M.
注意事项 Soluble in Water.
厂商提供的图像
 image for rCharybdotoxin (ABIN1001667) mSlo channel currents, before and during application of 100nM rCharybdotoxin. The cur...
背景 Meera, Wallner, Toro: "A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 10, pp. 5562-7, 2000 (PubMed).

有引用在: Vita, Bontems, Bouet, Tauc, Poujeol, Vatanpour, Harvey, Menez, Toma: "Synthesis of charybdotoxin and of two N-terminal truncated analogues. Structural and functional characterisation." in: European journal of biochemistry / FEBS, Vol. 217, Issue 1, pp. 157-69, 1993 (PubMed).

Gimenez-Gallego, Navia, Reuben, Katz, Kaczorowski, Garcia: "Purification, sequence, and model structure of charybdotoxin, a potent selective inhibitor of calcium-activated potassium channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 10, pp. 3329-33, 1988 (PubMed).

Smith, Phillips, Miller: "Purification of charybdotoxin, a specific inhibitor of the high-conductance Ca2+-activated K+ channel." in: The Journal of biological chemistry, Vol. 261, Issue 31, pp. 14607-13, 1986 (PubMed).

Miller, Moczydlowski, Latorre, Phillips: "Charybdotoxin, a protein inhibitor of single Ca2+-activated K+ channels from mammalian skeletal muscle." in: Nature, Vol. 313, Issue 6000, pp. 316-8, 1985 (PubMed).