rBmKTX

ABIN1001605 产品详细信息, 供应商: Log in to see
适用
Scorpion
宿主
大肠杆菌(E. Coli)
选项
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序列 VGINVKCKHS GQCLKPCKDA GMRFGKCING KCDCTPK.
特异性 Voltage-gated channel (Kv1.3) blocker. rBmKTX blocks Kv1.3 channels with IC50 of 019 nM.
产品特性 Source: Mesobuthus Martensii (Buthus martensii).
Molecular formula: C169H274N52O47S7.
Disulfide bonds between Cys7-Cys27, Cys13-Cys32, and Cys17-Cys34.
纯度 > 98 %
背景 BmKTX inhibits Kv1.3 channels heterologously expressed in HEK-293 cells. Kv1.3 channel currents, before and after application of 100pM BmKTX. Kv1.3 channel currents were elicited by depolarizing the voltage steps of 200 ms from the holding potential -80 mV to +50 mV.
分子量 3962
应用备注 0.1-1nM
说明

K+ channel toxin -KTx 3.6

限制 仅限研究用
状态 Lyophilized
溶解方式 Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.262ml will provide a stock solution of 10 M.
注意事项 Soluble in Water.
厂商提供的图像
 image for rBmKTX (ABIN1001605) Kv1.3 channel currents, before and after application of 100pM BmKTX. Kv1.3 channel cu...
有引用在: Renisio, Romi-Lebrun, Blanc, Bornet, Nakajima, Darbon: "Solution structure of BmKTX, a K+ blocker toxin from the Chinese scorpion Buthus Martensi." in: Proteins, Vol. 38, Issue 1, pp. 70-8, 2000 (PubMed).

Dai, Wu, Gu, Lan, Ling, Chi: "Genomic organization of three novel toxins from the scorpion Buthus martensi Karsch that are active on potassium channels." in: The Biochemical journal, Vol. 346 Pt 3, pp. 805-9, 2000 (PubMed).

Romi-Lebrun, Lebrun, Martin-Eauclaire, Ishiguro, Escoubas, Wu, Hisada, Pongs, Nakajima: "Purification, characterization, and synthesis of three novel toxins from the Chinese scorpion Buthus martensi, which act on K+ channels." in: Biochemistry, Vol. 36, Issue 44, pp. 13473-82, 1997 (PubMed).